A new study shows that glyphosate herbicide (e.g. Roundup) enhances the growth of aflatoxin-producing fungi.

GM Bt crops have long been touted as a solution to the problem of aflatoxins in grains. Aflatoxins are toxic and in some cases carcinogenic to animals and humans. The fungi that produce aflatoxins also cause plant diseases.
http://news.bbc.co.uk/1/hi/sci/tech/482467.stm
http://www.ncbi.nlm.nih.gov/pubmed/16779644

Oddly, however, aflatoxins were found last year at very high levels in US corn, even though most US corn is now Bt.
http://buzz.naturalnews.com/000633-corn-agriculture-aflatoxin.html
http://www.aganytime.com/Corn/Pages/Article.aspx?name=Aflatoxin-in-Corn-and-Crop-Insurance&fields=article&article=244

The key to this puzzle may lie in the fact that much Bt corn planted in the US is now also tolerant to Roundup herbicide.
http://www.agry.purdue.edu/ext/corn/news/timeless/BiotechTraits.html

Extrapolating from the new study's findings, applications of Roundup may increase the aflatoxin levels in the corn.

1. Study links Roundup 'weedkiller' to overgrowth of deadly fungal toxins
2. Influence of herbicide glyphosate on growth and aflatoxin B1 production by Aspergillus section Flavi strains isolated from soil on in vitro assay - study abstract
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1. Study links Roundup "weedkiller" to overgrowth of deadly fungal toxins
Sayer Ji
Greenmedinfo.com, 9 Sept 2013
http://www.greenmedinfo.com/blog/breaking-study-links-roundup-weedkiller-overgrowth-deadly-fungal-toxins-1
[references, useful charts, and links to sources at the URL above]

A new study reveals that Roundup herbicide enhances the growth of aflatoxin-producing fungi, lending an explanation for the alarming increase in fungal toxins recently discovered in U.S corn, and revealing another way in which GM farming is seriously undermining food quality.

A new study lead by Argentinean researchers and published in the Journal of Environmental Science and Health titled, "Influence of herbicide glyphosate on growth and aflatoxin B1 production by Aspergillus section Flavi strains isolated from soil on in vitro assay"[1], adds to an increasing body of research indicating that glyphosate (aka Roundup), the primary herbicide used in GM agriculture, is seriously undermining the quality of our global food supply, and may help to explain recent observations that GM corn heavy markets, such as the U.S., have a significant aflatoxin problem.[2]

Researchers from the Department of Microbiology and Immunology, National University of Rio Cuarto, Cordoba, Argentina, set out to evaluate the effect of glyphosate (Roundup) on the growth of aflatoxin B1 production by strains of Aspergillus under different water availabilities on maize based medium. Aflatoxin B1, one of at least 14 different types, is a naturally occurring mycotoxin that is produced by Aspergillus flavus and Aspergillus parasiticus, two species of fungi that commonly effect cereal grains. Known to be one of the most carcinogenic substances in existence, aflatoxin B1 is classified by the International Agency for Research on Cancer (IARC) as "Group 1, carcinogenic to humans," with an oral, rat LD50 (the dose that acutely kills 50% of a test group) of 5mg/kg – compare that to a 6.4 mg/kg LD50 for potassium cyanide, which is used in lethal injection.

The authors of the study pointed out that that little previous research has been performed on the role of glyphosate on the growth rate of aflatoxin-producing fungal species. The researchers also described the relevance this information has to the Argentinean corn market:

"Aspergillus section Flavi and Nigri Argentina is the world's second biggest exporter of maize (Zea mays L.), and was responsible roughly for 15 percent of the world's maize exports in the last three years. During the harvest season 2011/2012 the maize production is expected to be of 20 million tons. These cereal grains are colonize by several fungi communities, including mycotoxigenic species."

Argentina's total acreage dedicated to GM corn, while small in comparison to the U.S. majority stake in the world market, is second only to the U.S. [See figure 1]

Also, Argentina's GM corn share in the total GM corn acreage of their country is on par with the U.S. [see figured 2 below], indicating that their environmental and toxicological situation in regard to the food quality fallout from GM farming is likely very similar.

Researchers Discover Roundup Enhances Growth of Aflatoxin-Producing Fungi

In brief, the researchers discovered that all six different concentrations of glyphosate tested decreased the lag phase of fungi growth proportionately to the increase in glyphosate concentrations. In other words, the glyphosate enhanced the growth of the aflatoxin-producing Apergillus strains, and at concentrations lower than the range generally detected in Argentinean soils destined to crop production, specifically an agricultural area belonging to the province of Buenos Aires.[3]

In the author's words:

"This study has shown that the eight Aspergillus flavus and A. parasiticus strains evaluated are able to grow effectively and produce AFs [aflatoxins] in natural medium with high nutrient status over a range of glyphosate concentrations under different aW [water activity] conditions."

The figure below shows the influence of glyphosate on growth and aflatoxin B1 production…

The discovery that glyphosate enhances fungal growth contradicts several previous studies, including a 2007 study performed by US Department of Agriculture researchers[4], which did not find that glyphosate increased Aspergillus flavus growth. The authors noted that their findings are consistent with research on similar fungal strains, such as Fusarium[5], which possesses high tolerance to applied doses of glyphosate, and Rust fungi and Blight fungi[6], [7] which exhibit enhanced growth on glyphosate-amended media.

They noted: "[S]everal studies have demonstrated that microbial activity and/or biomass can be stimulated following application of some glyphosate formulation to field soil." This may be explained by the fact that glyphosate-tolerant species of fungi use glyphosate as a source of 'food,' utilizing available phosphate or amine structures that result from its metabolic breakdown. Indeed, previous studies indicate glyphosate can be used by fungal strains as a "nutriment" and "energy substrate."[8],[9],[10]

The Toxicological Nightmare of GM Food Grows Darker

A major implication of the study is that there exists a synergism between glyphosate (Roundup) and soil-borne pathogens, which would lead to increased susceptibility to and severity of disease in glyphosate-treated plants.[11] Not only would Roundup-ready corn contain residues of highly toxic glyphosate, its 'inactive' yet still highly toxic ingredients (surfactants), and metabolites (AMPA), but it would also be more likely to contain aflatoxins – taken together, represent a veritable nightmare of synergistic toxicities, whose sum harms no regulatory agency yet adequately accounts for.

The researchers conclude their paper with a cautionary note: "This situation suggests that quantitative changes could occur in these fungi population in the soil exposed to longtime action of this xenobiotic. The survival of these microorganisms, capable to adapt to different glyphosate concentration represents a toxicological risk..."

When one takes into account recent research that Roundup herbicide contributes to the suppression of beneficial lactic-acid producing gut bacteria, while enhancing some of the most deadly known to man, e.g. Clostridium botulinum (1 kilogram (2.2 lbs) would be enough to kill the entire human population), the days of casually classifying the ever-expanding numbers of anti- or non-GMO supporters and activists as alarmists, or GM food itself as "substantially equivalent" to non-GM food, are over. Those who continue to toe Biotech's party-line, under the much maligned banner of checkbook "Science," and in face of clear evidence against its safety, will increasingly be perceived as morally, financially and even legally liable for the damages being caused to exposed populations.

[1] Carla L Barberis, Cecilia S Carranza, Stella M Chiacchiera, Carina E Magnoli. Influence of herbicide glyphosate on growth and aflatoxin B1 production by Aspergillus section Flavi strains isolated from soil on in vitro assay. J Environ Sci Health B. 2013 ;48(12):1070-9. PMID: 24007484

[2] Reuters.com, Tate & Lyle says aflatoxin in U.S. corn complicates grain sourcing, Nov. 2012

[3] Peruzzo, P.; Porta, A.; Ronco, A. Levels of glyphosate in surface waters, sediments and soils associated with direct sowing soybean cultivation in north pampasic region of Argentina. Environ. Pollut. 2008, 156, 61–66.

[4] K N Reddy, H K Abbas, R M Zablotowicz, C A Abel, C H Koger. Mycotoxin occurrence and Aspergillus flavus soil propagules in a corn and cotton glyphosate-resistant corn.

[5] Larson, R.L.; Hill, A.L.; Fenwick, A.; Kniss, A.R.; Hanson, L.E.; Miller, S.D. Influence of glyphosate on Rhizoctonia and Fusarium root rot in sugar beet. Pest. Manag. Sci 2006, 62, 1182–1192.

[6] Wardle, D.A.; Parkinson, D. Influence of the herbicide glyphosate on soil microbial community structure. Plant Soil 1990, 122, 29–37.

[7] Hanson, K.G.; Fernandez, M.R. Glyphosate herbicides affect plant pathogenic fungi. Can. J. Plant. Pathol 2003, 25, 120.

[8] Krzysko-Łupicka, T.; Orlik, A. The use of glyphosate as the sole source of phosphorus or carbon for the selection of soil-borne fungal strains capable to degrade this herbicide. Chemosphere 1997, 34, 2601–2605.

[9] Krzysko-Łupicka, T.; Strof, W.; Kubs, K.; Skorupa, M.; Wieczorek, P.; Lejczak, B.; Kafarski, P. The ability of soil-borne fungi to degrade organophosphonate carbon-to-phosphorus bonds. Appl. Microbiol. Biotechnol 1997, 48, 549–552.

[10] Krzysko-Lupicka, T.; Sudol, T. Interactions between glyphosate and autochthonous soil fungi surviving in aqueous solution of glyphosate. Chemosphere 2008, 71, 1386–1391.

[11] Rahe, J.E.; Levesque, C.A.; Johal, G.S. Synergistic role of soil fungi in the herbicidal effect of glyphosate. In Microbes and microbial products as herbicides, : Hoagland, R.E., Ed.; ACS Symposium Series No. 439; Oxford University Press: New York, 1990; 260–275.

2. Influence of herbicide glyphosate on growth and aflatoxin B1 production by Aspergillus section Flavi strains isolated from soil on in vitro assay
Barberis CL, Carranza CS, Chiacchiera SM, Magnoli CE.
J Environ Sci Health B. 2013;48(12):1070-9. doi: 10.1080/03601234.2013.824223.
http://www.ncbi.nlm.nih.gov/pubmed/24007484

Source
Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico, Químicas y Naturales , Universidad Nacional de Río Cuarto, Córdoba, Argentina.

Abstract

The effect of six glyphosate concentrations on growth rate and aflatoxin B1 (AFB1) production by Aspergillus section Flavi strains under different water activity (aW) on maize-based medium was investigated. In general, the lag phase decreased as glyphosate concentration increased and all the strains showed the same behavior at the different conditions tested. The glyphosate increased significantly the growth of all Aspergillus section Flavi strains in different percentages with respect to control depending on pesticide concentration. At 5.0 and 10 mM this fact was more evident; however significant differences between both concentrations were not observed in most strains. Aflatoxin B1 production did not show noticeable differences among different pesticide concentrations assayed at all aW in both strains. This study has shown that these Aspergillus flavus and A. parasiticus strains are able to grow effectively and produce aflatoxins in high nutrient status media over a range of glyphosate concentrations under different water activity conditions.